Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Hepatoprotective effect of desi and kabuli cultivars of Cicer arietinum L (chick peas) against carbon tetrachloride-induced toxicity in rats

Mudasir Majeed¹, Abdullah Ijaz Hussain² , Haseeb Anwar², Shahzad Irfan³, Shahzad Ali Shahid Chatha², Qasim Ali⁴, Imran Mukhtar³, Zahra Hafeez⁵

¹Department of Applied Chemistry; ²Natural Product and Synthetic Chemistry Laboratory, Department of Chemistry; ³Department of Physiology; ⁴Department of Botany, Government College University; ⁵Department of Botany, University of Agricultural, Faisalabad, Pakistan.

For correspondence:- Abdullah Hussain Email: abdullahijaz@gcuf.edu.pk Tel:+923007631058

Accepted: 22 February 2020 Published: 31 March 2020

Citation: Majeed M, Hussain AI, Anwar H, Irfan S, Chatha SA, Ali Q, et al. Hepatoprotective effect of desi and kabuli cultivars of Cicer arietinum L (chick peas) against carbon tetrachloride-induced toxicity in rats. Trop J Pharm Res 2020; 19(3):609-615 doi: 10.4314/tjpr.v19i3.22

© 2020 The authors.
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Abstract

Purpose: To determine the hepatoprotective potential of ethanol extracts of desi and kabuli cultivars of Cicer arietinum L. (chick peas).

Methods: Hepatotoxicity was induced in rats using oral administration of carbon tetrachloride (CCl₄). The rats were then orally administered different doses of the ethanol extracts of desi and kabuli cultivars of Cicer arietinum L. for 21 days. Oxidative stress parameters and hepatoprotective profiles were determined in serum samples using standard procedures. The effect of the treatments on liver histology was also determined.

Results: Administration of extracts of desi and kabuli cultivars of Cicer arietinum L. to CCl₄ treated rats at a dose of 300 mg/kg resulted in a significant (p ≤ 0.05) decrease in oxidative stress parameters, whereas catalase activity significantly increased (p ≤ 0.05); on the other hand, ALT and AST levels were decreased significantly (p ≤ 0.05), when compared to the control group.

Conclusion: High doses of Cicer arietinum L (desi and kabuli cultivars) seem to have hepatoprotective and antioxidant effects on CCl₄-induced toxicity in rats. This finding underscores the therapeutic importance of Cicer arietinum L. as a plant with hepatoprotective properties.

Keywords: Cicer arietinum, Phenolics, Hepatotoxicity, Chick peas, Catalase